Programmable
DNA Lattices: Design Synthesis and Applications
Duke
University (John H Reif, Principal
Investigator)
Contract Quadchart: [ ppt
] [ pdf
]
Duke
University STATEMENT OF WORK
We
will make an experimental demonstration and
comparison of a number of methods for assembly of complex patterns,
these include:
- unmediated algorithmic
self-assembly (programmed assembly via Wang tiles), and
- step-wise assembly, and our
directed nucleation assembly technique(where an input DNA strand is
synthesized that encodes the required pattern, and then specified tiles
assemble around blocks of this input DNA strand, forming the required 1D
or 2D pattern of tiles).
We will to demonstrate algorithmic self-assembly of DNA and
controlled nucleation of self-assembled structures for some complex designs and
will experimentally determine error rates during unmediated self-assembly.
We will design and characterize various novel DNA tiles, and
apply these to generate various DNA tilings.
- For
example, we will modify the TX molecules with additional Holiday junctions
between the top and bottom dsDNA, so the resulting tile, a “Cylindrical
TX tile” (CTX), has a cylindrical conformation. We will characterize
1 D tilings of CTX tiles; we expect these form long cylinders which may be
able to capture linear structures (e.g., carbon nanotubes).
- We
will further investigate the use of these and related families of DNA
tiles to form 3D DNA lattices based on hybrids of CTX plus more
conventional DX and TX tiles.
We will also provide overall administration of the contract
research.
Also, see
NYU
Statement of Work
Caltech
Statement of Work